Product Name                                                                                   Anti B Monoclonal Antibodies

Brand                                                                                         J MITRA & CO PVT LTD 

Pack Size                                                                                              10 ml

Catalog No.                                                                                         BR240010

Type:                                                                                               Blood Grouping Reagent

Intend Use:                                 Anti-B Monoclonal Antibodies is an in-vitro culture supernatant of hybrids obtained by cellular fusion (Hybridoma Technology).

Intended Use

Anti-B Monoclonal Antibodies is an in-vitro culture supernatant of hybrids obtained by cellular fusion (Hybridoma Technology). It is a monoclonal antibody for ABO grouping. The reagent is for in-vitro use only.

Principle

The ABO Grouping is defined both by the presence of ‘A’ and/or B’ antigens on the surface of red blood cells and by the simultaneous presence of anti-A and/or anti-B antibodies in the serum. An individual has in his serum the antibodies corresponding to the antigens which are not on his red blood cells. It is thus necessary to identify the erythrocyte antigens by the known ANTI- A, ANTI- B and ANTI- AB monoclonal antibodies (forward typing) and then confirm the results by verifying the presence of corresponding antibodies in the blood serum to be tested with control cells: ‘A1’, ‘A2‘, ‘B’ and ‘O’ (Reverse typing). Human red blood cells possessing ‘A’ and /or ‘B’ antigen will agglutinate in the presence of antibody directed towards the antigen. For example, agglutination of RBCs with Anti –B monoclonal antibodies indicates the presence of ‘B’ Antigen on the red blood cells.

Cells                                                                 Avidity                                                      Intensity                                        Titre

B                                                                       2-4 seconds                                               ++++                                               =256

A1B                                                                   5-6 seconds                                               ++/+++                                            =256